The Eppendorf 5402 is a compact, electronic single-channel pipette widely used for life‑science labs requiring precise low-volume liquid handling (0.5–10 µL, 2–20 µL, 10–100 µL, or 20–200 µL models). An updated manual clarifies operation, maintenance, calibration, troubleshooting, and safety to reduce user error and extend instrument life.
| Code | Probable Cause from Community Reports | Suggested Actions | | :--- | :--- | :--- | | | Rotor not recognized or speed measurement system failure. | Check rotor is correctly installed and recognized by the centrifuge. | | Error 1 | Rotor identification failure. | Ensure rotor is properly installed; if issue persists, contact support. | | ERR 02 | Rotor imbalance or over-speed detection. | Ensure rotor is properly seated and loaded with balanced tubes. | | Error 3 | Incorrect speed measurement method. | Check speed sensor and its connections. | | Error A | Temperature sensor failure. | Requires sensor replacement; parts may be limited. |
Adjustable from 0 to 14,000 RPM in 100 RPM increments. Maximum RCF: Up to Temperature Control: A broad cooling range from -9∘Cnegative 9 raised to the composed with power C 40∘C40 raised to the composed with power C
The Eppendorf Centrifuge 5402 remains a classic workhorse in molecular biology, biochemistry, and clinical laboratories worldwide. Renowned for its robust build and dependable refrigerated performance, this microcentrifuge is essential for pelleting nucleic acids, spinning down proteins, and preparing clinical samples. eppendorf 5402 manual updated
Regular preventative maintenance extends the lifespan of the Eppendorf 5402 and protects laboratory personnel from biohazardous contamination. Daily Maintenance Tasks
The is not a myth, but it is fragmented. Here is your immediate action plan:
Sets the duration of the run. Turning the knob fully counter-clockwise or past 99 minutes engages the continuous "Hold" mode. The Eppendorf 5402 is a compact, electronic single-channel
Press STOP immediately. Wait for standstill. Clean spilled liquids and re-balance tubes using a scale. Condenser coils blocked; lid seal leaking; low refrigerant.
is often described as an "old school" workhorse. Its brushless, maintenance-free motor design contributes to its longevity in high-throughput environments .
1 to 99 minutes, or continuous operation (Hold) 2. Installation and Safety Guidelines | Check rotor is correctly installed and recognized
Ultimate Guide to the Eppendorf 5402 Centrifuge: Operation, Maintenance, and Troubleshooting
Turn on the main power switch located on the side or rear panel of the unit.
Press the Stop key to manually terminate a run. The braking system will engage to bring the rotor to a smooth halt.
If the unit displays errors like Error 3 or Error 23 , it typically indicates the system is not detecting the rotor or there is a drive recognition fault; restarting the device often resolves minor glitches.